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Starter Flat-lined: Advice?

Valdus's picture
Valdus

Starter Flat-lined: Advice?

I have a ten year old starter I got from a baker friend at the Farmers Market here in Gretna, LA. I always had my starter in the fridge, never a problem- even after hurricanes.

This time I took out my starter, fed it, and waited.

The starter, after 12 hours, smelled and looked exactly like it was supposed to.

But there was no rise, no life, with few bubbles.

Note: The only thing I did that might have damaged it, is I put it out on the windowsill. While there is no direct sunlight, it still is damn bright.

I have struggled with bringing it to life for over two weeks. I even "rebooted" it, using a tiny amount of starter and discarding and feeding as if it was a new starter.

So far nothing, smells great, absolutely great, but absolutely no rise, no doubling, nothing goes over the rubber band.

Help! This starter is over 10 years old and would prefer to save it.

One starter I used AP Flour (Arrowhead) the other one I used 50/50 whole wheat/bread flour.

Both are flatlined.

PS: I have a dried backup starter powder. But that behaved the same when I tried it a week ago. 

I tried another dehydrated starter I once made, 12 hours- nothing, using whole wheat flour. 

Should I continue these experiments or start a new starter? I also have a lot of discard from when I first took it out of the fridge.

Thanks for any suggestions you may have, 

 

DanAyo's picture
DanAyo

How long had it been since the refrigerated starter was fed?

Valdus's picture
Valdus

I have been feeding and discarding for about 2 weeks. I have three samples currently. They are there smelling like wondrous lacto roses. 

stiff starter has some rise. Clone has some hooch.  

miiiiiiight be good. 

phaz's picture
phaz

Get a dough consistency - wait till it thins out -  repeat. When it takes a day or so to thin out it's ready for use. Enjoy!

Valdus's picture
Valdus

Not sure what you mean by consistency or which starter. I have three experiments going. A new one from dried starter, a stiff one with wheat, and another with AP flour that is rather flat. 

phaz's picture
phaz

Consistency = how it feels. Will work for any starter. Enjoy!

mariana's picture
mariana

Would you please describe how you refresh your starter(s) exactly, Valdus? Proportions, temperature?

For sourdough yeast to proliferate and produce gas (leaven the dough) it needs three things

1) optimal temperature (28-32C),

2) optimal nutrition (3% sugar, NA flours have 0% sugar and not all flours are malted, have enzymes added by millers) and

3) optimal refreshment periods, not too short and not too long. If you refresh twice a day at 27C, for example, the best schedule is 8hrs+ 16hrs, i.e.one refreshment favors bacteria and another - yeasts.

Some sourdough yeasts cannot tolerate cold. That was the case of San-Francisco sourdough starter when it was discovered by scientists in the seventies. When refrigerated, it would simply flatline. The myths about it being a local culture which was unable to syrvive in other cities proliferated. But that was not the case. Its yeast would die unable to survive at the temperatures below 8-10C, i.e. refrigerated. So in between bakes it had to be kept either on its owner's body as gold prospectors had it or at least in a cool but not cold place (10-12C).

Some sourdough yeasts do not survive either drying or rehydration step, if it is not done correctly (1hr at 40C, then for 24-48hrs at 30C). Even baker's yeast strains are like that, for example, active dry yeast strain has to be rehydrated at 38-42C first, even room temperature water kills it, let alone cold water, whereas instant dry yeast strain is OK even with ice cold water.

So you either had few if any living cells left in your refrigerated and dried samples, or they did not survive the refreshment process, or your refreshment process does not allow them to thrive and grow in numbers.

I'll share my experiences with yeastless starters, I had a couple like yours in the past, and what helped to restore or reintroduce sd yeast in them, but first please describe your feeding ratios and temperature at which your starters stand in between refreshments. 

Valdus's picture
Valdus

I feed my starter, either whole wheat or whole wheat/ bakers flour and keep it at a constant 82 degrees F. I do it about every twelve or twenty four hours. I just did it this morning and the dried starter, discard or stiff starter have not risen from the respective line. 

My ratio has been usually 30-30-35. 

Abe's picture
Abe

They've been fed now so don't do anything more till they show signs of life. If you continue to discard and feed before they've re-activated, you'll be throwing away all the good stuff and you'll end up with plain flour and water. 

Instead of feeding just give them a stir for now. 

Valdus's picture
Valdus

Not a feeding. I just stirred it and changed the top to a paper towel and rubber band for more oxygen. 

Will wait…

mariana's picture
mariana

Ok, thanks!

To restore your dried starter, if you have a portion of dried starter left, take a small amount of dried flakes, add to them a small pinch of sugar or a drop of honey and distilled water (105F/40C) to make a paste, and keep it covered at 82F/28C for 48hours (full two days), undisturbed. Then refresh/feed it bread flour and water, cover and keep it undisturbed for 24hrs at 28C/82F. Then it should be ready to use in baking.

If you have no dried starter left, you can do the same with your reconstituted from dried starter: 3% sugar added to it, flour or bran added to it to make a stiff paste, covered and left for 48hrs at 82F/28C. 

You say that one of them is a stiff starter, however that is not possible with 30-30-35. 

Anyways, what helped me with a white nearly dead  100% hydration starter was to feed it with a scald, either all of the flour in the feeding portion was scalded (mixed with water 1f:3w and heated to 160F/70C, until it thickens) or only half of it, let it cool then mix this scald (and raw flour if you wish) with your starter, 3% sugar, and enough water to mimic 100% hydration dough.

The flour in the scald should be at least a shade darker than the bread/all-purpose flour that you are using now, i.e higher extraction. You can add a pinch of bran, crushed bran cereal, or whole grain flour to bread flour if you do not have slightly higher extraction flours at home, like type 650-750 flours.

The starter woke up and bubbled like crazy immediately (overnight).Scalded and sweetened higher extraction flours have a magical effect on liquid starters, mostly because they provide yeasts with all the necessary nutrition in extracted (dissolved) and easily digested (cooked) form.

What helped me with a stiff white flour starter that smelled amazing of sourdough, like lactic roses as you said, but was not risng at all, was to prepare a portion of "liquid sourdough yeast" and use it to feed the starter.

 Fragrant and sour but yeastless starter that never rose. I refrigerated it for three days, while I was preparing "liquid sourdough yeast" for it, to introduce species of SD yeasts into my starter. Bubbles on its surface are due to kneading, trapped air, not to alcoholic fermentation.

 Liquid sourdough yeast.

For that I mixed 6 cups of water, a heaping  spoon of spelt flour, a heaping spoon of that yeastless but sour stiff starter, and a few squished raw grapes for nutrition. Covered it and let it stand for three days undisturbed. The first two days it smelled like kefir or yogurt, later, as a bowl of champagne, full of sourdough yeasts gassing.

 Ready to use liquid sourdough yeast. It can be slightly sweetened and kept for a long time refrigerated. 

I used that liquid, strained , and a portion of whole wheat flour to feed that refrigerated white stiff starter. Of course, it rose, doubling, and then I switched to only water and bread flour.

. The first feeding of the refrigerated flat starter with that liquid yeast and whole wheat flour doubled.

The following feedigs with water and bread/all-purpose flours gave a normal  100%hydration white sourdough starter, that quadruples in volume in 6 hours or less at 82F/27C and stands tall for hours without deflating much (my flours are fortified with vitamin C, that helps).

 

Valdus's picture
Valdus

You have given me a wealth of knowledge to choose from. 

tpassin's picture
tpassin

This is all extremely interesting and is another illustration that there are many, many ways to producing a starter.  I still am not convinced, though, that the original flat starter was brought back to life - as opposed to a new starter having been created.  I don't know how one could tell, without being able to analyze the actual organisms, if that was the case.  I suppose one could go through all the same steps without including bits of the old flat starter and see if things evolved differently.

I recently got a new starter going beginning with "beaten rice".  Beaten rice is white rice that has been smashed flat into flakes, sometimes with and sometimes without a heat treatment.  I covered some of the flakes with water, and when that had been absorbed added more water until the flakes remained covered by the water.  I covered the bowl with plastic wrap and left it on the kitchen counter.  Nothing seemed to be happening until day 3.  Then it had a somewhat fruity smell, one I had never smelled from a starter before.

At that point I added some white flour and adjusted the water so the mixture was still rather liquidy.  I stirred it from time to time through all this. The next day the fruity odor was gone, the day after that a few bubbles appeared, the next day still more.  I may have fed it with more white flour and water at this point but I can't remember for sure.  The next day, day 7, bingo! It appeared to be a normal white flour starter, and it could leaven dough very well.

My point is not that it's a good method to start with beaten rice.  It's just that that there are so many ways to get a starter going (or maybe reconstituted) that it's hard to know if the reconstitution really happened.  For my own purposes, I assume that if I can wake up a starter within two or maybe three days then it has probably been awakened from dormancy, but if it takes nearly a week it might as well be a new starter since one can be created from scratch in that time.  And anyway, if it takes so much manipulation to re-awaken, then the starter's properties will have been changed so much that it might as well be a new one anyway.

Valdus's picture
Valdus

How well do you cover the yeast water for the 3 days? 

mariana's picture
mariana

I cover it with a shower cap, Or a plastic shopping bag. I.e.it lets air circulate as water is self-mixing because it is so liquid and releases CO2 and aromas into the headspace. Otherwise the cover will explode under gas pressure. 

Here the important thing not to miss is its alcoholic smell when it appears because, if you do not add some sugar (3% re: weight of water in the cup) at that point, the yeasts will stop working and it will take quite a while to wake them up again (over 12 hours), the yeast water will not be immediately useful. 

So, measure its temperature regularly and sniff it from time to time to not miss that moment, when it smells clearly alcoholic and, if stirred or shaken, behaves like coca-cola or champagne when those are shaken or stirred. 

 

Valdus's picture
Valdus

So, wait even past the 24 hour mark? 

Abe's picture
Abe

Yes... even past the 24 hour mark. 

If you see a small amount of activity then you can give them a small feed. If it picks up then increase the feeds. Feed according to the strength of the starter. 

Valdus's picture
Valdus

No disrespect to others’ suggestion but our own Dan suggested simply a 15/15/15 discard, 120 F water, with wheat flour and see if there is any activity in the 4, 8 or 12 hours. 

This might be Angela’s last chance. 

Experiment 4

 

Valdus's picture
Valdus

By the way, I did a slightly smaller (3cups) version of Mariana’s Franken-Yeast-Water. 

I just couldn’t resist; it’s so experimental! 

Valdus's picture
Valdus

Next day, absolutely nothing on all fronts. I guess Ill just wait for the yeast water. 

Abe's picture
Abe

What do they smell like? 

Valdus's picture
Valdus

They smell like the first day of the world- great. Lacto goodness. 

Is this a sign that I ran out of yeast beasts?

Abe's picture
Abe

Smelling yoghurty is a good sign. 

Valdus's picture
Valdus

Its getting to the 24 hour mark since last feed 24 hours at 10 am

Abe's picture
Abe

Give them a good stir and wait a further 12-24 hours. Waiting 2 days when a starter goes quiet is not out of the ordinary. 

If you really want to do some sort of feed then I believe you have 3 experiments going so give one of them a small feed. Just a little extra water and some of the rye you have bought. Not too much! And no need to discard. 

Valdus's picture
Valdus

I bought some rye flour, would that make a difference?

Valdus's picture
Valdus

no rise, no feedings, just a stir. Stuff has about three bubbles and very liquidy.

Rye flour and raisin yeast water (2 forms) coming up. 

squattercity's picture
squattercity

did you try the rye flour. I've found rye to be the key to my sourdough starter -- super active and very accommodating of time in the refrigerator.

Rob

Valdus's picture
Valdus

It is coming tomorrow. I am going to drizzle my whole house in rye. 

squattercity's picture
squattercity

covering everything with rye is highly recommended!

Valdus's picture
Valdus

It is Day 4 on this strange odyssey of starter (though now it seems more like finisher). Upon waking there was no rise, smell good, very yogurty smell. Main starter jar had three bubbles. 

At this point I am waiting on the rye and still fermenting raisins. One water is covered loosely the other in a jar, but the other has not hissed. Loose one is 3 days, Jar is 1 day. 

I think I am going to use this post to document this strange, strange flat-line journey. 

Note: I emailed Mauricio, the perfect loaf blogger. He suggested discarding and feeding once a day.

With respect, I had done that and may have diluted the starter altogether. 

Thoughts?

squattercity's picture
squattercity

how old is the flour you are using?

In my experience, old flour can stunt yeast growth, even if the date on the bag says it is still usable.

Rob

Valdus's picture
Valdus

The flour isn’t new but not old either. I bought new bread flour and rye cometh today. 

squattercity's picture
squattercity

could your water be an issue?

Valdus's picture
Valdus

I use the water from my fridge; it has a filter. 

Valdus's picture
Valdus

Rye flour here. Raisin water sitting for 3 days.

Also, one of my discard jars has hooch! Does that mean it’s active? 

So, do I use that hooches discard for the starter? Raisin waters? 

combos? 

mariana's picture
mariana

Valdus, although rye flour is notoriously low in yeasts, sometimes to the point of lacking them, it might contain enough wild yeast cells to help you. So, add 1 spoon of rye flour to your liquid yeast in the making, please. It won't hurt and it might help.

NO RAISINS, please, it must be unwashed and squished fresh grapes + spelt four + your starter and water held at 90 degrees F.

With chopped raisins, if you are not lucky, it would take up to 10 days at 82 degrees F for the yeast to grow. It happened to me once. It resulted in a good starter, but the method was unacceptably long. 

Hooch is the sign of gluten rot. Freshly mixed dough absorbs water very well and with time it absorbs more and more until a maximum amount of gluten forms and flour proteins bind the maximum amount of water to them. With time, as gluten ages, proteins begin release water. That is what phaz called "thinning out" when he told you to track your starter's quality in his advice to you above and not to refresh too soon.

A good starter has microbes that protect gluten, so it does not thin out even in 24 hours at warm room temperature. It holds water bound to its proteins so well. Starters in their early stages have none of those bacteria and tend to become gluey, thinner, or release water very soon after refreshment. 

Assuming that your discards have good lactic bacteria in them, true sourdough bacteria, the hooch is the sign of the gluten rot due to time. Normally, hooch would be alcoholic, very boozy, if your sourdough starter has yeasts in them. If it is simply acidic, then no yeast, simply water and acids released by the dough as its protein matrix ages. 

 

Valdus's picture
Valdus

I think I know what I did but….

It was like mixing a dough, you keep going, you keep going, then suddenly WHAM it all comes together. I think the infusion of rye, fine fine rye, that did it. 

mariana's picture
mariana

Congratulations, Valdus! Well done! Thank you for the update. 

squattercity's picture
squattercity

fantastic! Rye rules!

Valdus's picture
Valdus

I got back into baking and damn if it didn’t happen again. Damn if I can’t remember what I did last time! 

Dave Cee's picture
Dave Cee

Just try bottled water, one time.

 

Valdus's picture
Valdus

I will try that because now it has officially flat-lined. I will try Ed Wood's wash and bottled water. Perhaps with a bit of rye.